| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Effects of oleic acid on the high threshold barium current in seabass Dicentrarchus labrax ventricular myocytes
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| Autores: |
Chatelier, Aurelien
Imbert, Nathalie
Zambonino, Jose-luis
Mckenzie, David
Bois, P
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| Data: |
2006-10
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| Ano: |
2006
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| Palavras-chave: |
Fatty acid
Sea bass
Ventricular myocyte
L type calcium channel
Oleic acid
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| Resumo: |
The present study employed a patch clamp technique in isolated seabass ventricular myocytes to investigate the hypothesis that oleic acid (OA), a mono-unsaturated fatty acid, can exert direct effects upon whole-cell barium currents. Acute application of free OA caused a dose-dependent depression of the whole-cell barium current that was evoked by a voltage step to 0 mV from a holding potential of -80 mV. The derived 50% inhibitory concentration (IC50) was 12.49 +/- 0.27 mu mol l(-1). At a concentration of 30 mu mol l(-1), OA significantly reduced the current density to about 45% of control values, but did not modify either the shape of the current-density voltage relationship or the apparent reversal potential. In addition, OA did not modify the voltage dependence of either steady state inactivation or activation curves. Taken together, these results indicate that physiological concentrations of free OA decrease the conductance of the L-type inward current, without altering its properties of selectivity and its voltage dependence. The inhibitory effect of OA upon the L-type calcium channel may translate, in vivo, into a protective effect against arrhythmias induced by Ca2+ overload.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2006/publication-2120.pdf
DOI:10.1242/jeb.02470
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| Editor: |
The Company of Biologists
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| Relação: |
http://archimer.ifremer.fr/doc/00000/2120/
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| Formato: |
application/pdf
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| Fonte: |
Journal of Experimental Biology (0022-0949) (The Company of Biologists), 2006-10 , Vol. 209 , N. 20 , P. 4033-4039
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| Direitos: |
The Company of Biologists 2006
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